Mechanosensitive Membrane Probes
نویسندگان
چکیده
منابع مشابه
Headgroup engineering in mechanosensitive membrane probes.
Systematic headgroup engineering yields planarizable push-pull flipper probes that are ready for use in biology - stable, accessible, modifiable -, and affords non-trivial insights into chalcogen-bond mediated mechanophore degradation and fluorescence enhancement.
متن کاملFluorescent Flippers for Mechanosensitive Membrane Probes
In this report, "fluorescent flippers" are introduced to create planarizable push-pull probes with the mechanosensitivity and fluorescence lifetime needed for practical use in biology. Twisted push-pull scaffolds with large and bright dithienothiophenes and their S,S-dioxides as the first "fluorescent flippers" are shown to report on the lateral organization of lipid bilayers with quantum yield...
متن کاملMechanosensitive membrane channels in action.
The tension-driven gating process of MscL from Mycobacterium tuberculosis, Tb-MscL, has been addressed at near-atomic detail using coarse-grained molecular dynamics simulations. To perform the simulations, a novel coarse-grained peptide model based on a thermodynamic parameterization of the amino-acid side chains has been applied. Both the wild-type Tb-MscL and its gain-of-function mutant V21D ...
متن کاملGating-by-tilt of mechanosensitive membrane channels
We propose an alternative mechanism for the gating of biological membrane channels in response to membrane tension that involves a change in the slope of the membrane near the channel. Under biological membrane tensions we show that the energy difference between the closed (tilted) and open (untilted) states can far exceed k B T and is comparable to what is available under simple dilational gat...
متن کاملActivation of mechanosensitive currents in traumatized membrane.
Mechanosensitive (MS) channels, ones whose open probability varies with membrane tension in patch recordings, are diverse and ubiquitous, yet many are remarkably insensitive to mechanical stimuli in situ. Failure to elicit mechanocurrents from cells with abundant MS channels suggests that, in situ, the channels are protected from mechanical stimuli. To establish what conditions affect MS channe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemistry - A European Journal
سال: 2015
ISSN: 0947-6539
DOI: 10.1002/chem.201406320